R26STOPrtTA Mouse Strain- a Unique System for Spatiotemporal and Lineage-specific Gene Expression

Brief Description

Applications:

Inventor has developed an advanced method for conditional gene expression in mice that integrates the Cre-mediated and tetracycline-dependent expression systems. An rtTA gene, preceded by a loxP-flanked STOP sequence, was inserted into the ROSA26 locus to create a R26STOPrtTA mouse strain. When the STOP sequence is excised by Cre-mediated recombination, the rtTA is expressed in the Cre-expressing cells and all of their derivatives. Therefore, cell type-, tissue-, or lineage-specific expression of rtTA is achieved by the use of an appropriate Cre transgenic strain. In mice also carrying a target gene under the control of the tetracycline response element, inducible expression of the target gene is temporally regulated by administration of doxycycline.

Advantages:

Results demonstrate that this universal system is uniquely suited for spatiotemporal and lineage-specific gene expression in an inducible fashion. Gene expression can be manipulated in specific cell types and lineages with a flexibility that is difficult to achieve with conventional methods. This mouse model has been well characterized in H-M Ivy Yu, Bo Liu, Shang-Yi Chiu, Frank Costantini and Wei Hsu (2005). Development of a unique system for spatiotemporal and lineage-specific gene expression in mice. Proc Natl Acad Sci USA, 102, 8615-8620.

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Daisy Bunbury
Licensing Manager
University of Rochester
 
Inventors:
Wei Hsu
Keywords: