Stable Human Fc Receptor-Transformed Cell Lines to Detect Virus-Neutralizing and Enhancing Antibodies

Brief Description
Applications:
The invention offers a novel method to screen anti-flavivirus antibodies in blood for neutralizing and replication-enhancing antibodies using Fc gamma R transformed macrophages.  Although this invention is immediately applicable to dengue virus infection, in principle it is applicable to any viral pathogen whose replication may be enhanced by antibodies.

Advantages:
The invention offers simplicity and the prospect of an automated, high through-put alternative to both conventional virus plaque neutralization and immune enhancement assay. The method will offer a standardized format to replace current laborious methods to measure protective neutralizing and potentially undesirable immune-enhancing antibodies stimulated by natural infection or by candidate and established viral vaccine.
Patent Information:
Title Country Patent No. Issued Date
Method of Determining Immune Enhancement of Virus Infectivity using FC Receptor-Transfected Cell Lines United States 8,592,163 11/26/2013
Category(s):
Vaccines
For Information, Contact:
Daisy Bunbury
Licensing Manager
University of Rochester
 
Inventors:
Jacob Schlesinger
Robert Rose
W.W. Shanaka Rodrigo
Xia Jin
Keywords:
Vaccine