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Use of a Heme Tag for Expressed Protein Purification, Visualization, and Quantitation
The heme tag is a heme convalently bound to a peptide appended to a protein of interest. It's used for 1) affinity purification of a protein. 2) Visual tracking of a protein during expression and purification 3) quantization of a protein using absorption spectroscopy 4) determination of a protein?s extinction coefficient at 280nm.Applications:This protein...
Published: 10/7/2021
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Inventor(s):
Kara Bren
,
Wesley Asher
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Research Tools