Identification of Hairpin DNA Probes by Partial-Gene Folding

Brief Description

Brief Description: This disclosure describes a new method of molecular beacon discovery which relies on the generation of naturally occurring hairpins.


Applications:  The use of DNA hairpins as molecular beacons, both in solution, and immobilized on a solid surface, has proven to be an excellent method for ?label-free? detection of biological entities. This invention will be of use in biosensing, genetic analysis, pharmaceutical development, medical research, and rapid medical diagnostics.


Advantages:   The traditional method of molecular beacon generation is to supplement a naturally occurring DNA sequence at both the 5? and the 3? ends with the necessary nucleotide composition to force the formation of a hairpin.  This technique has a major flaw in that the introduction of nucleotides that are not specific for the intended target sequence increases the likelihood of non-specific binding, or, even worse, selective binding to the wrong target.  The use of naturally occurring DNA hairpins obviates this flaw by eliminating the need for supplementation of additional bases, the result: a probe that is completely specific for its designed target.

Patent Information:
Title Country Patent No. Issued Date
Method of Identifying Hairpin DNA Probes by Partial Fold Analysis United States 7,598,034 10/6/2009
Method of Identifying Hairpin DNA Probes by Partial Fold Analysis United States 7,442,510 10/28/2008
For Information, Contact:
John FahnerVihtelic
Senior Licensing Manager
University of Rochester
Benjamin Miller
Christopher Strohsahl