A Cell Line Capable of Sensing HIF-1alpha Activation in the CNS for Use in Screening Pharmacologic Agents Capable of Activating/Attenuating Hypoxic Activated Gene Transcription

Brief Description
Applications:
This technology describes a stable reporter cell line that measures the expression of hypoxia-inducible-1-alpha (HIF-1a), a transcriptional activator, which is useful in screening compound libraries for anti-ischemic, neuroprotective and tumor pro-apoptotic activity. Drug candidates identified with this technology could potentially address therapies for central nervous system ischemia, cardiac ischemia, and cancer.

Advantages:
This cell lines offer a fast and economical tool to learn how drug leads can regulate pathologic hypoxia-dependent transcriptional processes such as: delayed neuronal death which occurs following CNS ischemia, myocyte death caused by recurrent myocardial ischemia, and hypoxic signaling which contributes to the progression of solid tumors.
  
Patent Information:
Category(s):
Research Tools
For Information, Contact:
Daisy Bunbury
Licensing Manager
University of Rochester
 
Inventors:
Howard Federoff
Marc Halterman
Keywords:
Cell Line
Drug screening