Description: The present
technology relates to hybridization-based biosensors containing hairpin probes
and their use in identifying target nucleic acids in samples.
Applications: This label-free
microarray will be used as a rapid genetic analysis tool for understanding
biological processes. It has immediate applications in medical diagnostics,
biosensors, genome analysis, etc.
Advantages: Recent intense
interest in the use of rapid genetic analysis as a tool for understanding
biological processes, in unlocking the underlying molecular causes of disease,
and in the development of biosensors, has led to a need for new sensitive and
arrayable chip-based analytical tools. Of high importance is the need for
techniques that do not require labeling of the target sample, since that
increases time, cost, and potential for error inherent in the analysis. The
label-free biosensor discussed herein presents a more efficient, sensitive and
cost effective way to identify and study target nucleic acids in samples. Most
notably, this novel approach uses the substrate itself as the quenching agent,
which significantly decreases the complexity of the synthetic DNA probe hairpin,
by eliminating the need for a separate quencher.